A Comparative Study on Rapid, Simple and Cost-Effective Method in Determining the Decalcification Agent in Bone Tissue Processing in Hematoxylin and Eosin Staining Procedure

Vinegar is most commonly used as a condiment with food.however, there are also different types of vinegar that are very effective for cleaning.In addition to household vinegar, it is mainly produced as a precursor to polyvinyl acetate and cellulose acetate.In our present invention we have used vinegar as decalcification agent in bone specimen in the hematoxylin and eosin staining .The present study, apple vinegar used as decalcifying agent in processing bone specimens for hematoxylin and eosin staining.A study was conducted from apiril 2022 to September 2022 , 30 bone specimens were taken from department of pathology .Bone Sections were made into the block and sent for the Hematoxyline and eosin staining .In H&E staining two groups were separated Group A , Group B, group C and group D .Group A used vinegar as decalcifying agent ,Group B as 10% HCL, group C as 2% NO and sodium bicarbonate solution as decalcifying agent ,thin section were made 3-4 micron and bone sections were subjected to hematoxyline and eosin staining All group slides were given to the pathologist for they opinion and for reporting the slides.The results showed that Group A and group C had showed superior staining properties ,.cost effective decalcification agent was sodium bicarbonate and vinegar when compared to the other two.The study suggest that vinegar is consider as ecofriendly decalcifying agent in hematoxyline and eosin staining process.