Avoiding overflow metabolite formation in Komagataella phaffii fermentations to enhance recombinant protein production
These findings underscore the importance of a suitable glucose-feeding strategy in K. phaffii fermentation processes and highlight the detrimental effects of overflow metabolites on productivity. By optimizing carbon source utilization, it is possible to enhance fermentation efficiency and recombinant protein production with K. phaffii.
Authors
- Jørgen Barsett Magnus (ORCID: https://orcid.org/0009-0007-4381-0088)
- Lars M. Blank (ORCID: https://orcid.org/0000-0003-0961-4976)
- Jochen Büchs (ORCID: https://orcid.org/0000-0002-2012-3476)
- Marcel Mann
- Monica I. Espinosa (ORCID: https://orcid.org/0000-0001-6200-7089)
- Thomas Steimann (ORCID: https://orcid.org/0009-0009-9429-7741)
- Judith Wegmann
Institutions
- RWTH Aachen University (DE)
Publication Details
- Journal
- Journal of Biological Engineering
- Published
- 2024-10-03
- DOI
- https://doi.org/10.1186/s13036-024-00453-0
- Citations
- 8
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 1.87
Funders
- RWTH Aachen University