Metabolic Reprogramming of <i>Klebsiella pneumoniae</i> Exposed to Serum and Its Potential Implications in Host Immune System Evasion and Resistance
The aim of this study was to identify, using proteomics, the molecular alterations caused by human serum exposure to <i>Klebsiella pneumoniae</i> ACH2. The analysis was performed under two different conditions, native serum from healthy donors and heat-inactivated serum (to inactivate the complement system), and at two different times, after 1 and 4 h of serum exposure. More than 1,000 bacterial proteins were identified at each time point. Enterobactin, a siderophore involved in iron uptake, and proteins involved in translation were upregulated at 1 h, while the chaperone ProQ and the glyoxylate cycle were identified after 4 h. Enzymes involved in the stress response were downregulated, and the SOD activity was validated using an enzymatic assay. In addition, an intricate metabolic adaptation was observed, with pyruvate and thiamine possibly involved in survival and virulence in the first hour of serum exposure. The addition of exogenous thiamine contributes to bacterial growth in human serum, corroborating this result. During 4 h of serum exposure, the glyoxylate cycle (GC) probably plays a central role, and the addition of exogenous succinate suppresses the GC, inducing a decrease in serum resistance. Therefore, serum exposure causes important changes in iron acquisition, the expression of virulence factors, and metabolic reprogramming, which could contribute to bacterial serum resistance.
Authors
- Lucélia Santi (ORCID: https://orcid.org/0000-0002-5698-7006)
- Markus Berger (ORCID: https://orcid.org/0000-0001-6676-6090)
- Jorge A. Guimarães (ORCID: https://orcid.org/0000-0001-6354-6789)
- Rafael Lopes da Rosa (ORCID: https://orcid.org/0000-0002-8283-2833)
- John R. Yates (ORCID: https://orcid.org/0000-0001-5267-1672)
- Patricia Orlandi Barth (ORCID: https://orcid.org/0000-0003-0763-1430)
- Walter O. Beys‐da‐Silva (ORCID: https://orcid.org/0000-0001-8329-7839)
- Amanda Naiara Silva Moraes
- Franciele Maboni Siqueira (ORCID: https://orcid.org/0000-0001-7282-6117)
- Afonso Luís Barth (ORCID: https://orcid.org/0000-0002-7969-3908)
- Juliana Miranda Tatara (ORCID: https://orcid.org/0000-0001-8524-0158)
- Guilherme Domingues
Institutions
- Universidade Federal do Rio Grande do Sul (BR)
- Scripps Institution of Oceanography (US)
- Universitas Borneo Tarakan (ID)
- Institute of Experimental Physics of the Slovak Academy of Sciences (SK)
- Hospital de Clínicas de Porto Alegre (BR)
- Experimental Station (US)
- Hospital Ernesto Dornelles (BR)
- Czech Academy of Sciences, Institute of Experimental Medicine (CZ)
- CNR de la Résistance aux Antibiotiques (FR)
- National Institute of Allergy and Infectious Diseases (US)
- Scripps (United States) (US)
- Cellular Research (United States) (US)
- Respiratory and Meningeal Pathogens Research Unit (ZA)
- University of Rio Grande and Rio Grande Community College (US)
Publication Details
- Journal
- Journal of Proteome Research
- Published
- 2024-10-03
- DOI
- https://doi.org/10.1021/acs.jproteome.4c00286
- Citations
- 3
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 0.51
Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul
- National Institute of Allergy and Infectious Diseases