A molecular comparison of [Fe-S] cluster-based homeostasis in <i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i>

ISC and SUF molecular systems build and transfer Fe-S cluster to cellular apo protein clients. The model <i>Escherichia coli</i> has both ISC and SUF and study of the interplay between the two systems established that the ISC system is the house-keeping one and SUF the stress-responding one. Unexpectedly, our recent phylogenomic analysis revealed that in contrast to <i>E. coli</i> (and related enterobacteria such as Salmonella), most bacteria have only one system, and, in most cases, it is SUF. <i>Pseudomonas aeruginosa</i> fits the general rule of having only one system but stands against the rule by having ISC. This study aims at engineering <i>P. aeruginosa</i> harboring <i>E. coli</i> systems and vice versa. Comparison of the recombinants allowed to assess the functional versatility of each system while appreciating their contribution to cellular homeostasis in different species context.

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Publication Details

Journal
mBio
Published
2024-10-03
DOI
https://doi.org/10.1128/mbio.01206-24
Citations
5
Primary Topic
Metalloenzymes and iron-sulfur proteins
Type
article
Field-Weighted Citation Impact
0.51

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article

A molecular comparison of [Fe-S] cluster-based homeostasis in Escherichia coli and Pseudomonas aeruginosa

Pierre Garcia, Francesco Imperi, Emmanuel Séchet, Frédéric Barras et al.
5 citations
mBio
Metalloenzymes and iron-sulfur proteins
0.51
article

A molecular comparison of [Fe-S] cluster-based homeostasis in Escherichia coli and Pseudomonas aeruginosa

Pierre Garcia, Francesco Imperi, Emmanuel Séchet, Frédéric Barras, Shirley Genah, Maria Concetta Spinnato, Francesca D’Angelo, Ehud Banin, C. C. Matteo, Alessandra Lo Sciuto
article en
5 citations

Abstract

ISC and SUF molecular systems build and transfer Fe-S cluster to cellular apo protein clients. The model <i>Escherichia coli</i> has both ISC and SUF and study of the interplay between the two systems established that the ISC system is the house-keeping one and SUF the stress-responding one. Unexpectedly, our recent phylogenomic analysis revealed that in contrast to <i>E. coli</i> (and related enterobacteria such as Salmonella), most bacteria have only one system, and, in most cases, it is SUF. <i>Pseudomonas aeruginosa</i> fits the general rule of having only one system but stands against the rule by having ISC. This study aims at engineering <i>P. aeruginosa</i> harboring <i>E. coli</i> systems and vice versa. Comparison of the recombinants allowed to assess the functional versatility of each system while appreciating their contribution to cellular homeostasis in different species context.

mBioVol. 15(11)
Roma Tre University (IT), Centre National de la Recherche Scientifique (FR), Bar-Ilan University (IL), Institut Pasteur (FR), Université Paris Cité (FR), Fondazione Santa Lucia (IT), Istituti di Ricovero e Cura a Carattere Scientifico (IT), University of Palermo (IT)
European Commission, Agence Nationale de la Recherche, Istituto Pasteur-Fondazione Cenci Bolognetti, CHIST-ERA
Life in Land
Openalex Percentile: Top 43%
Metalloenzymes and iron-sulfur proteins
0.51
DOIOpen Access PDFOpenAlex
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