Integrating the Transcriptome and Proteome to Postulate That TpiA and Pyk Are Key Enzymes Regulating the Growth of Mycoplasma Bovis

<i>Mycoplasma bovis</i> is a global problem for the cattle industry due to its high infection rates and associated morbidity, although its pathophysiology is poorly understood. In this study, the <i>M. bovis</i> transcriptome and proteome were analyzed to further investigate the biology of clinical isolates of <i>M. bovis</i>. A differential analysis of <i>M. bovis</i>, a clinical isolate (NX114), and an international type strain (PG45) at the logarithmic stage of growth, was carried out using prokaryotic transcriptome and 4D-label-free quantitative non-labeled proteomics. Transcriptomics and proteomics identified 193 DEGs and 158 DEPs, respectively, with significant differences in 49 proteins/34 transcriptomic CDS post-translational protein sequences (15 jointly up-regulated and 21 jointly down-regulated). GO comments indicate membrane, cytoplasmic and ribosome proteins were important components of the total proteins of <i>M. bovis</i> NX114 clinical isolate. KEGG enrichment revealed that <i>M. bovis</i> NX114 is mainly associated with energy metabolism, the biosynthesis of secondary metabolites, and the ABC transporters system. In addition, we annotated a novel adhesion protein that may be closely related to <i>M. bovis</i> infection. Triosephosphate isomerase (TpiA) and Pyruvate kinase (Pyk) genes may be the key enzymes that regulate the growth and maintenance of <i>M. bovis</i> and are involved in the pathogenic process as virulence factors. The results of the study revealed the biology of different isolates of <i>M. bovis</i> and may provide research ideas for the pathogenic mechanism of <i>M. bovis</i>.