Adlercreutzia wanghongyangiae sp. nov., and Adlercreutzia shanghongiae sp. nov., two new members of the genus Adlercreutzia isolated from plateau pika (Ochotona curzoniae)
Four anaerobic, Gram-stain-positive, non-motile, non-sporulating rod-shaped bacterial strains (R7<sup>T</sup>, R21, R22 and R25<sup>T</sup>) were isolated from the intestinal contents of plateau pika (<i>Ochotona curzoniae</i>) collected from the Qinghai-Tibet Plateau, PR China. The four isolates grew at between 25 and 42 °C (optimally at 35-37 °C), and with 0.3-3.3% NaCl (w/v) [optimum, 1.3% (w/v)]. Adding l-arginine to the medium could promote their growth. Strains R7<sup>T</sup> and R21 were most closely related to <i>Adlercreutzia caecimuris</i> B7<sup>T</sup> (97.48% 16S rRNA gene sequence similarity). Strains R25<sup>T</sup> and R22 were most closely related to <i>Adlercreutzia</i> e<i>quolifaciens</i> DSM 19450<sup>T</sup> (98.25% 16S rRNA gene sequence similarity). The genome sequences of R7<sup>T</sup> and R25<sup>T</sup> were 2.89 and 2.90 Mb in size with 63.6 and 62.8 mol% DNA G+C contents, respectively. Phylogenetic analysis based on 16S rRNA gene sequences and core genes revealed that R7<sup>T</sup> and R21 were most closely related to <i>A. caecimuris</i> B7<sup>T</sup> and <i>Adlercreutzia mucosicola</i> DSM 19490<sup>T</sup>, whereas R25<sup>T</sup> and R22 were most closely related to <i>A. equolifaciens</i> DSM 19450<sup>T</sup> and <i>Adlercreutzia rubneri</i> ResAG-91<sup>T</sup>. R7<sup>T</sup>, R25<sup>T</sup> and the closely related species had average nucleotide identity (ANI) values of 81.9-83.2% as well as digital DNA-DNA hybridisation (dDDH) values between 27.3 and 27.9%, which clearly indicated that they represent two novel species within the genus <i>Adlercreutzia</i>. For R7<sup>T</sup> and R25<sup>T</sup>, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan, and the whole cell sugars included galactose, glucose and ribose. On the basis of these results, we propose that strains R7<sup>T</sup> and R25<sup>T</sup> represent two novel species of the genus <i>Adlercreutzia,</i> namely <i>Adlercreutzia wanghongyangiae</i> sp. nov. and <i>Adlercreutzia shanghongiae</i> sp. nov., respectively. The type strains are R7<sup>T</sup> (=GDMCC 1.4459<sup>T</sup>=KCTC 25860<sup>T</sup>) and R25<sup>T</sup> (=GDMCC 1.4458<sup>T</sup>=KCTC 25861<sup>T</sup>).
Authors
- Liyun Liu (ORCID: https://orcid.org/0000-0001-6397-7693)
- Jianguo Xu (ORCID: https://orcid.org/0000-0003-3649-8229)
- Huimin Zhou (ORCID: https://orcid.org/0000-0002-3736-1745)
- Ji Pu (ORCID: https://orcid.org/0000-0003-2268-9796)
- Caixin Yang
- Lin Ye (ORCID: https://orcid.org/0000-0003-0062-6436)
- Xiaorui Liu (ORCID: https://orcid.org/0009-0001-8258-5457)
- Jing Yang (ORCID: https://orcid.org/0000-0002-5865-3165)
- Linglin Cao
- Shuo Ning
- Yuyuan Huang (ORCID: https://orcid.org/0009-0005-3406-4875)
Institutions
- Shanxi Medical University (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- National Institute for Communicable Disease Control and Prevention (CN)
- Hebei Provincial Center for Disease Control and Prevention (CN)
Publication Details
- Journal
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
- Published
- 2024-10-03
- DOI
- https://doi.org/10.1099/ijsem.0.006531
- Citations
- 1
- Primary Topic
- Cocoa and Sweet Potato Agronomy
- Type
- article
- Field-Weighted Citation Impact
- 0.86
Funders
- National Center of Competence in Research Materials’ Revolution: Computational Design and Discovery of Novel Materials