Characteristics of organic amendments induce diverse microbial metabolisms for exogenous C turnover in Mollisols

The response of microbial community to organic amendments is closely related to the amendment-derived carbon (C) turnover, which in turn influences the enhancement of soil organic C, including the quantity and quality. The objective of this study was to quantify the decomposition and retention of exogenous C derived from amendments, and to identify the microbial regulatory mechanisms of exogenous C turnover in response to the characteristics of organic amendments. The 13 C-labelled glucose, straw and biochar, characterizing organic amendments with high, medium and low labilities, respectively, were used to conduct a 90-day incubation experiment, in conjunction with stable isotope probing technology and amino sugar molecular biomarkers. It was found that the cumulative mineralization of amendment-derived C increased with increasing the corresponding lability of organic amendment, and the decompositions of the added glucose, straw and biochar, expressed as a percentage of the initially added C, were 53.6 %, 37.0 %, and 0.6 %, respectively. Among all treatments, the addition of straw significantly enhanced the total dissolved organic C (DOC) concentration and its ratio to total organic C by increasing the input of exogenous DOC and reducing the decomposition of native soil DOC. The exogenous C use efficiency by microorganisms was significantly enhanced with decreasing the amendment lability, and showed a positive correlation with the total amendment-derived C. However, the microbial growth efficiency was the highest when straw was added, and was closely related to the concentration of amendment-derived DOC. Different functional groups of soil microbes exhibited distinct capacities to metabolize various organic amendments. Generally, the incorporation of amendment-derived C into microbial biomass was significantly affected by fungi and gram-positive (G + ) bacteria, while the relative contributions of microbial communities incorporating amendment-derived C into necromass were decreased in the order of gram-negative (G − ) bacteria > fungi > actinomycetes > G + bacteria. From the perspective of microbial metabolism, the larger incorporations of exogenous C into microbial biomass and necromass were observed in the soil with mediumly labile amendment, which contributed to enhance the quantity and quality of organic C in soil. These results would be informative to optimize the organic amendment strategy for integrated soil fertility management. • The lability of organic amendment-derived C regulates its microbial metabolism. • Labile amendments decompose quickly and contribute little to soil organic C quantity. • Stable amendments have a high CUE but contribute little to soil organic C quality. • Exogenous C incorporations into microbial PLFAs affect organic C quantity and quality.