Antihypertensive, anti-inflammatory, glycaemic, and satiety effects of cocoa-flavoured soy-based powder drink mix enriched with bioactive hydrolysates from nests of <i>Aerodramus fuciphagus</i>

Abstract Objectives Edible bird nest hydrolysates (EBNHs) are renowned for their health benefits. The study aimed to determine their functional effects as an ingredient in cocoa-flavoured soy-based powder drink mix (PDM). The glycaemic and satiety effects of the product were also studied. Materials and Methods The product was enriched with a reasonably low amount of spray-dried EBNH. The product’s functional effects with respect to in vitro angiotensin-converting enzyme (ACE) inhibitory activity and human red blood cell membrane stabilization (RBCMS) activity were evaluated using its water extract (90–95 °C). The glycaemic and satiety impacts were evaluated in twelve and thirty subjects, respectively, who consumed 250 mL of the product, which contained 25 g of available carbohydrates. Results The enriched PDM ACE inhibitory activity and RBCMS activity were significantly higher (P&amp;lt;0.05) than those that were devoid of EBNH. Two hundred and forty milliliters (12.5% (125 mg/mL)) of PDM (containing 3.0% EBNH, 75.44% soybean powder, 20% cocoa powder, 0.5% xanthan gum, 1.0% silicon dioxide, and 0.06% sucralose (mass fraction)) provides approximately 3.7 kcal/g energy. The 250 mL meal (consistency index of 14.17 Pa·sn) is high in protein and dietary fibre. The product’s glycaemic index (GI) and glycaemic load (GL) are low, 24 and 6.0, respectively, raising a normal level of 2-h postprandial blood glucose (&amp;lt;6 mmol/L) and satisfying hunger for approximately 2.5 h. Conclusions EBNH, as an ingredient, enhances the antihypertensive and anti-inflammatory properties of PDM. The overall findings suggest that the formulated PDM is a wellness food product capable of promoting good human health, including for dieters and individuals with diabetes.