Manipulation and characterization of brain cells using multimodal optical force microscopy

Brain cells are complex and delicate materials with unique physical properties that can be influenced by physical stress. Recent studies have shown that physical stress affects the biological response of molecules inside living cells. We have been developing a single-objective light-sheet microscope and optical manipulation tools to investigate the dynamic properties of living brain cells. This integration allows us to observe 3D biological processes with high precision while enabling the manipulation and characterization of individual cells and microcompartments. In this presentation, I will explain the operating principles of multimodal optical force microscopy, share preliminary results, and discuss future applications.