Combined optical trapping and fluorescence for single T cell activation profiling

T cell clones can exhibit varying potencies towards their cognate peptide major histocompatibility complex molecule (pMHC), which may not be seen in traditional bulk assays. Performing single molecule and single cell experiments, including profiling through Single Cell Activation Requirements allow for further discrimination and selection of the highest quality clones. Using these techniques will help reveal how T cells function and advance screening methods for selecting the best T cells to use therapeutically.