Nano-optical trapping and detection of nanoscale biological particles using all-dielectric nanoantenna

We present a method for trapping nanoscale biological particles without causing photothermal heating, using all-dielectric optical nanotweezers. This technique focuses the accessible electromagnetic energy to as small as 30 nm, enabling the trapping and imaging of nanosized biological specimens including extracellular vesicles and lipoproteins with minimal temperature increase. This advancement offers new opportunities in single molecule analysis and opens the door to understanding the diversity of extracellular particles.