Transient absorption and phase microscopy with balanced detection

This presentation focuses on the use of common path, birefringent, time-delayed interferometry to detect ultrafast pump-probe effects via phase, rather than absorption of the probe pulse, in a laser-scanning microscope. The method uses balanced detection to cancel relative intensity noise inherent to fiber laser sources. We compare absorption and phase measurements in graphene, hemoglobin, and red blood cells, and present a preliminary model for determining whether, for a given wavelength, absorption or phase will yield a stronger signal.

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Published
2024-10-02
DOI
https://doi.org/10.1117/12.3028248
Primary Topic
Advanced Fluorescence Microscopy Techniques
Type
article
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article

Transient absorption and phase microscopy with balanced detection

Jesse W. Wilson, Randy A. Bartels, Cameron N. Coleal
Advanced Fluorescence Microscopy Techniques
article

Transient absorption and phase microscopy with balanced detection

Jesse W. Wilson, Randy A. Bartels, Cameron N. Coleal
article en

Abstract

This presentation focuses on the use of common path, birefringent, time-delayed interferometry to detect ultrafast pump-probe effects via phase, rather than absorption of the probe pulse, in a laser-scanning microscope. The method uses balanced detection to cancel relative intensity noise inherent to fiber laser sources. We compare absorption and phase measurements in graphene, hemoglobin, and red blood cells, and present a preliminary model for determining whether, for a given wavelength, absorption or phase will yield a stronger signal.

Morgridge Institute for Research (US), Colorado State University (US)
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Advanced Fluorescence Microscopy Techniques
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