Análisis de la expresión del gen TRI5 de Fusarium graminearum en coleóptilos de trigo y maíz.

The necrotrophic fungus F. graminearum is responsible for causing Fusarium head blight in wheat and ear rotin maize. This fungal infection leads to reduced crop yields and contamination of the crops with mycotoxinslike trichothecene deoxynivalenol (DON), which can cause poisoning in animals and humans. The productionof DON is facilitated by a set of genes, with the TRI5 gene encoding trichodiene synthase being a key player.While some factors that can activate the TRI5 gene in wheat are known, it's unclear whether this gene isactivated when the fungus infects wheat or maize coleoptiles. This study reveals that using ammonium sulfateas a nitrogen source induces the expression of the TRI5 gene in minimal media. In wheat coleoptiles, F.graminearum forms infection structures at 5 days post-infection (dpi), where the induction of the TRI5 geneoccurs. On the other hand, there is no TRI5 gene induction in maize coleoptiles. Finally, the TRI5 geneexpression in plants was confirmed using semi-quantitative PCR, validating the induction in wheat coleoptilesat 5 dpi.