An atlas of the aging mouse proteome reveals the features of age-related post-transcriptional dysregulation
To what extent and how post-transcriptional dysregulation affects aging proteome remains unclear. Here, we provide proteomic data of whole-tissue lysates (WTL) and low-solubility protein-enriched fractions (LSF) of major tissues collected from mice of 6, 15, 24, and 30 months of age. Low-solubility proteins are preferentially affected by age and the analysis of LSF doubles the number of proteins identified to be differentially expressed with age. Simultaneous analysis of proteome and transcriptome using the same tissue homogenates reveals the features of age-related post-transcriptional dysregulation. Post-transcriptional dysregulation becomes evident especially after 24 months of age and age-related post-transcriptional dysregulation leads to accumulation of core matrisome proteins and reduction of mitochondrial membrane proteins in multiple tissues. Based on our in-depth proteomic data and sample-matched transcriptome data of adult, middle-aged, old, and geriatric mice, we construct the Mouse aging proteomic atlas ( https://aging-proteomics.info/ ), which provides a thorough and integrative view of age-related gene expression changes.
Authors
- Gregory Tombline
- Jun Adachi (ORCID: https://orcid.org/0000-0003-1220-3246)
- Vera Gorbunova (ORCID: https://orcid.org/0000-0001-8979-0333)
- Naoko Ohtani (ORCID: https://orcid.org/0000-0001-8934-0797)
- Kazuaki Takemura
- Masaki Takasugi (ORCID: https://orcid.org/0000-0001-9880-1225)
- Yuya Yoshida (ORCID: https://orcid.org/0000-0003-2394-9748)
- Jennifer Schwarz (ORCID: https://orcid.org/0000-0002-2406-0185)
- Frank Stein (ORCID: https://orcid.org/0000-0001-9695-1692)
- Andrei Seluanov (ORCID: https://orcid.org/0000-0003-3400-538X)
- Yoshiki Nonaka
- Seyed Ali Biashad
- Junko Satoh
- Shinji Ito
Institutions
- European Molecular Biology Organization (DE)
- Osaka Ohtani University (JP)
- Kyoto University (JP)
- National Institute of Biomedical Innovation, Health and Nutrition (JP)
- Osaka Metropolitan University (JP)
- University of Rochester (US)
- Tokyo Metropolitan University (JP)
Publication Details
- Journal
- Nature Communications
- Published
- 2024-10-02
- DOI
- https://doi.org/10.1038/s41467-024-52845-x
- Citations
- 31
- Primary Topic
- Single-cell and spatial transcriptomics
- Type
- article
- Field-Weighted Citation Impact
- 7.25
Funders
- Suzuken Memorial Foundation
- Nakatomi Foundation
- Mizutani Foundation for Glycoscience
- Japan Agency for Medical Research and Development
- University of Tokyo
- Astellas Pharma
- Astellas Foundation for Research on Metabolic Disorders
- Senri Life Science Foundation
- Chugai Pharmaceutical
- Japan Society for the Promotion of Science
- Japan Science and Technology Agency
- Precursory Research for Embryonic Science and Technology