MODIFICATION OF GLASS IONOMER FILLING MATERIAL BY METAL NANOPARTICLES ANDTHEIR COMPOUNDS: EFFECT ON ANTI-MICROBIAL ACTIVITY

Background: Glass ionomer filling materials are widely used in stomatology, but do not have an antimicrobial effect that effectively prevents the development of secondary and recurrent dental caries. Researchers are attempting to modify these materials by introducing various biologically active additives into them. The aim of the study was to evaluate the antimicrobial properties of a dental ionomer filling material modified withadditives based on high–energy nanoparticles of silver, copper, titanium and their compounds. Material and methods: Colloidal aqueous and alcoholic solutions of metals and their oxides with stabilizers were obtained by the electroerosion method. Citric acid, cetylpyridinium chloride and Trilon B were used as stabilizers. The zeta potential and the distribution of particles of the dispersed phase in solutions were measured. Samples of dental glass ionomer cement "Cemion-Aqua" were impregnated with colloidal solutions of nanoparticles. The microbiological activity of glass ionomer fillings samples in relation to plaque was determined by disc diffusion and suspension methods. Results: The results showed that modification of glass ionomer cement samples with silver hydrosols in citric acid solutions with concentrations of 0.04% and 0.0025% increases the zone of radial lysis of microbial plaque colonies around the cement samples by 1.5 and 2.5 times compared with the control. By the suspension method, it was determined that silver hydrosols in a solution of citric acid and without it reduce the formation of colonies of microorganisms to several units up to 72 hours of exposure compared with the control. And copper hydrosols in solutions of cetylpyridinium chloride prevent an increase in the number of colonies of microorganisms after 24 hours of exposure compared with the control. Silver hydrosol in a solution of citric acid with a concentration of 0.0025% and silver alcohol sol reduce the number of colonies of microorganisms to several units after 3 hours of exposure.