Plate centrifugation enhances the efficiency of polyethylenimine-based transfection and lentiviral infection
We present a protocol based on plate centrifugation for transfection or lentiviral infection that is suitable for genetic modification of primary cells or stem cells.
Authors
- Yuan Zhuang (ORCID: https://orcid.org/0000-0003-1696-1378)
- Junfeng Li (ORCID: https://orcid.org/0000-0001-6739-7805)
- Shaozhe Yang (ORCID: https://orcid.org/0009-0009-7306-827X)
- Xiuhong Fu (ORCID: https://orcid.org/0009-0004-4326-6113)
- Qingwei Zhang (ORCID: https://orcid.org/0000-0001-6302-2424)
Institutions
- Luohe Medical College (CN)
Publication Details
- Journal
- Journal of Virological Methods
- Published
- 2024-09-30
- DOI
- https://doi.org/10.1016/j.jviromet.2024.115039
- Citations
- 1
- Primary Topic
- Virus-based gene therapy research
- Type
- article
- Field-Weighted Citation Impact
- 0.55