Fine mapping of RNA isoform diversity using an innovative targeted long-read RNA sequencing protocol with novel dedicated bioinformatics pipeline
We have validated a new protocol for the enrichment of transcripts of interest using probes adapted to the ONT and PacBio platforms. This protocol allows a complete description of the alternative structures of transcripts, the estimation of their expression and the identification of aberrant transcripts in a single experiment. This proof-of-concept opens new possibilities for RNA structure exploration in both research and molecular diagnostics.
Authors
- Laurent Castéra (ORCID: https://orcid.org/0000-0002-0459-3400)
- Flavie Boulouard (ORCID: https://orcid.org/0000-0002-9923-0257)
- Dominique Vaur (ORCID: https://orcid.org/0000-0003-2792-8506)
- Nicolas Goardon (ORCID: https://orcid.org/0000-0002-5093-0922)
- Nicolas Soirat
- Philippe Denizeau
- Alexandre Atkinson (ORCID: https://orcid.org/0000-0003-2553-4925)
- Louise Crivelli
- Camille Aucouturier
- Céline Quesnelle
- Agathe Ricou (ORCID: https://orcid.org/0000-0002-8675-2961)
- Sophie Krieger (ORCID: https://orcid.org/0000-0001-7610-7355)
- Olivier Caron (ORCID: https://orcid.org/0000-0001-8934-2071)
- Angélina Legros (ORCID: https://orcid.org/0009-0004-9364-0134)
- Raphaël Leman (ORCID: https://orcid.org/0000-0003-1978-7133)
- Sosthène Barbachou
- Thibaut Lavolé
- Pascaline Berthet
- Julien Levilly
- Denis Bertrand
Institutions
- Inserm (FR)
- Institut Gustave Roussy (FR)
- Centre François Baclesse (LU)
- Quantum Genomics (France) (FR)
- Normandie Université (FR)
- Centre Eugène Marquis (FR)
- Centre François Baclesse (FR)
- Centre Hospitalier Universitaire de Rennes (FR)
- Université de Caen Normandie (FR)
Publication Details
- Journal
- BMC Genomics
- Published
- 2024-09-30
- DOI
- https://doi.org/10.1186/s12864-024-10741-0
- Citations
- 4
- Primary Topic
- RNA modifications and cancer
- Type
- article
- Field-Weighted Citation Impact
- 0.94