<scp>TMEM56</scp> deficiency impairs the haem metabolism and cell cycle progression during human erythropoiesis
TMEM56, a gene coding a transmembrane protein, is abundantly expressed in erythroid cells. Despite this, its role in erythropoiesis has not been well characterized. In this study, we sought to clarify the function of TMEM56 in erythroid development, focusing specifically on its involvement in haem biosynthesis and cell cycle progression. To do this, we used CD34<sup>+</sup> haematopoietic stem cells derived from umbilical cord blood and differentiated them into erythroid cells in an ex vivo model. Our results indicate that the loss of TMEM56 disrupts haem biosynthesis and impairs erythroid differentiation. Furthermore, deletion of Tmem56 in the erythroid lineage in murine models using erythropoietin receptor (EpoR)-Cre revealed defects in erythroid progenitors within the bone marrow under both normal conditions and during haemolytic anaemia. These observations underscore the regulatory role of TMEM56 in maintaining erythroid lineage homeostasis. Taken together, our results unveil a previously unrecognized function of TMEM56 in erythroid differentiation and suggest its potential as an unfounded target for therapeutic strategies in the treatment of erythropoietic disorders.
Authors
- Jie Gao (ORCID: https://orcid.org/0000-0002-4648-7956)
- Haoze Song (ORCID: https://orcid.org/0009-0000-5952-5168)
- Yipeng Liang
- Jinfa Ma (ORCID: https://orcid.org/0000-0002-2119-8779)
- Yanhong Zhao (ORCID: https://orcid.org/0009-0006-6387-0128)
- Lihong Shi (ORCID: https://orcid.org/0000-0001-8876-0802)
- Di Wang (ORCID: https://orcid.org/0000-0002-8904-2981)
- Yingnan Zhang (ORCID: https://orcid.org/0000-0002-2070-6786)
- Jing Liang (ORCID: https://orcid.org/0000-0002-0860-6563)
- Jinhua Liu (ORCID: https://orcid.org/0000-0002-4128-1043)
- Lingyue Zheng (ORCID: https://orcid.org/0009-0000-1060-632X)
- Zefeng Xu (ORCID: https://orcid.org/0000-0002-1581-7027)
- Yue Li (ORCID: https://orcid.org/0000-0001-6799-1812)
- Jin Xu (ORCID: https://orcid.org/0000-0002-9165-1962)
- Ding Wang (ORCID: https://orcid.org/0000-0002-6708-024X)
- Xiaoru Zhang (ORCID: https://orcid.org/0000-0002-8725-4091)
- Zhu Xu (ORCID: https://orcid.org/0000-0002-8057-7111)
- Lexuan Lin
- Jingwei Wang (ORCID: https://orcid.org/0009-0001-7657-6247)
- Qimei Cheng
- Jingyuan Tong
- Jing Wu (ORCID: https://orcid.org/0009-0004-6080-5908)
Institutions
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Institute of Hematology & Blood Diseases Hospital (CN)
Publication Details
- Journal
- British Journal of Haematology
- Published
- 2024-09-30
- DOI
- https://doi.org/10.1111/bjh.19801
- Primary Topic
- Erythrocyte Function and Pathophysiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- National Key Research and Development Program of China