DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR CONCURRENT DETERMINATION OF MUPIROCIN AND BECLOMETHASONE DIPROPIONATE IN PHARMACEUTICAL FORMULATION AND PERFORM FORCED DEGRADATION STUDY

Simple, specific, accurate, precise and reproducible method has been developed and validated for the simultaneous estimation of both drugs in their combined dosage form.In RP-HPLC, analysis is carried out using Methanol: Buffer pH 4 (65:35, v/v) mobile phase and Phenomenex Gemini ODS C18 column (200 mm x 4.6 mm, 5.0 particle size) as stationary phase with detection wavelength of 215 nm.Linearity was obtained in the concentration range of 40-120 g/ml and 0.5-1.5 g/ml for Mupirocin and Beclomethasone dipropionate respectively.The % recoveries of the both the drugs were found to be 99.45-99.86%and 100.10-100.39%respectively.LOQ were found to be 29.11g/ml and 0.297g/ml at 215 nm for Mupirocin and Beclomethasone dipropionate respectively.Methods were statistically validated for accuracy, precision, specificity, LOQ and robustness.Also carried out a Force degradation study according to ICH guidelines and can be used for analysis of combined dosage form.