Synthesis, Anticholinesterase and Antioxidant Activity of Thiosemicarbazone Derivatives

In this research, we report the synthesis and evaluation of novel thiosemicarbazones as anti-Alzheimer’s agents. The structural clarification of the newly synthesized compounds was carried out by 1H NMR, 13C NMR, and MS analyses. According to the in vitro cholinesterase inhibition assay, compounds showed more inhibitory potential against AChE than BuChE. The in vitro antioxidant activity of the synthesized compounds was measured via two different methods. According to ferrous ion-chelating assay compound 2b demonstrated 5.26% activity when compared to BHT (2.57%). DPPH radical scavenging activity assay revealed that compound 2b showed the most potent antioxidant activity with an IC50 value of 43.91 ± 0.021μM. Among the synthesized compounds, compound 2b was found as the most potent antioxidant agent.