A novel whole blood assay to quantify the release of T cell associated cytokines in response to Bordetella pertussis antigens
The developed WB-based cytokine assay was shown to be less costly, easy to perform, and functional in differently aged individuals. Further, it requires only a small amount of fresh blood, which is beneficial especially for studies including infants. Our results support the use of this assay for other immunological studies in the future.
Authors
- Anja Saso (ORCID: https://orcid.org/0000-0001-9130-543X)
- Cécile A. C. M. van (ORCID: https://orcid.org/0000-0003-1995-8641)
- Eleonora E. Lambert
- Elles Simonetti
- Alex‐Mikael Barkoff (ORCID: https://orcid.org/0000-0003-0742-1580)
- Elizabeth Clutterbuck (ORCID: https://orcid.org/0000-0003-1701-1390)
- Dimitri Diavatopoulos
- Jacqueline A. M. van Gaans-van den Brink
- Johanna Teräsjärvi (ORCID: https://orcid.org/0009-0008-8209-4384)
- Sophie Gimenez-Fourage
- Aymeric de Montfort
- Véronique Corbière
- Sophie Roetynck
- Pascal Blanc
- Aapo Knuutila (ORCID: https://orcid.org/0000-0003-0622-5043)
- Sagida Bibi (ORCID: https://orcid.org/0000-0002-0855-2737)
- Beate Kampmann (ORCID: https://orcid.org/0000-0002-6546-4709)
- Jussi Mertsola (ORCID: https://orcid.org/0000-0002-7746-3949)
- Marta Valente Pinto (ORCID: https://orcid.org/0000-0002-9716-7848)
- Dominic F. Kelly (ORCID: https://orcid.org/0000-0002-6063-8896)
- Anke Pagnon (ORCID: https://orcid.org/0000-0001-9295-3439)
- Qiushui He
- Haddijatou Jobe
Institutions
- Université Libre de Bruxelles (BE)
- Radboud University Nijmegen (NL)
- University of Turku (FI)
- Turku University Hospital (FI)
- Radboud University Medical Center (NL)
- Sanofi (France) (FR)
- Escola Superior de Saúde Egas Moniz (PT)
- University of Oxford (GB)
- National Institute for Public Health and the Environment (NL)
- Centre Léon Bérard (FR)
- Radboud Institute for Molecular Life Sciences (NL)
- MRC Unit the Gambia (GM)
- Berlin Institute of Health at Charité - Universitätsmedizin Berlin (DE)
- Oxford BioMedica (United Kingdom) (GB)
Publication Details
- Journal
- Journal of Immunological Methods
- Published
- 2024-09-30
- DOI
- https://doi.org/10.1016/j.jim.2024.113758
- Citations
- 2
- Primary Topic
- Bacterial Infections and Vaccines
- Type
- article
- Field-Weighted Citation Impact
- 0.69
Funders
- European Commission
- Sigrid Juséliuksen Säätiö
- Tampereen Tuberkuloosisäätiö
- Innovative Medicines Initiative