Fundamental Characteristics of Molecularly Imprinted Polymer Nanoparticles with High Affinity for Human Serum Albumin

In this research, the meticulous development and exhaustive analysis of molecularly imprinted polymer nanoparticles (nano-MIPs) are explored, with particular emphasis on their affinity for human serum albumin (HSA), a crucial biomarker for diagnosing type 2 diabetes mellitus.The yield of the synthesized nano-MIPs for HSA was determined by a freeze drying technique to be in the range of 6-15 mg for every 100 g of glass beads.A multifaceted characterization approach, including dynamic light scattering analysis, atomic force microscopy, and Fourier-transform infrared spectroscopy, was employed to rigorously assess the physicochemical properties of the nano-MIPs.The results indicate a relatively uniform size distribution and a well-defined chemical composition.Surface plasmon resonance kinetic analysis further revealed that the nano-MIPs exhibit high affinity and specificity for HSA, with a dissociation constant (Kd) of 2.37 10 -9 M and an R 2 value of 0.97.These findings not only validate the successful synthesis and characterization of nano-MIPs but also underscore their potential use for selective molecular recognition, thereby providing promising avenues for future research and applications in biomedical and environmental fields.