Tagging of Yellow Mosaic Virus resistance gene using SSR markers in Black gram (Vigna mungo L. Hepper)

Black gram is one of the most important pulse crops grown in India. The productivity of black gram is very low as it is cultivated in marginal lands with less care. Yellow mosaic virus disease in black gram is a serious concern as the yield reduction varies from 30 -100%. Breeding and cultivation of resistant varieties is the potential option to minimize yield reduction. However, the breakdown of resistance due to the evolution of new pathotypes in resistant varieties through conventional breeding leads to the adopting of biotechnological tools such as marker-assisted breeding. Identifying molecular markers linked with YMV resistance paved the way for changing black gram varieties with durable resistance. In the present study, 72 RILs developed by crossing the black gram lines ADT 3 and KKM 15052 as susceptible and resistant parents were used as mapping populations to tag YMV resistance with SSR markers. Among the 72 RILs screened for YMV resistance, 7 RILs (1,2,14,39,55,66 and 67) were resistant to YMV. Of 69 SSR markers, 14 were polymorphic between ADT 3 and KKM 15052. Single-marker analysis showed a significant association of the VR 086, VR 148 and CEDG 186 markers with YMV resistance. Bulked Segregant Analysis (BSA) confirmed that the SSR markers VR 046 and VR 148 are linked with the genes conferring resistance to YMV in KKB 15052.