Protocol to generate, purify, and analyze antibody-oligonucleotide conjugates from off-the-shelf antibodies
Antibody-oligonucleotide conjugates (AOCs) are a fast-expanding modality for targeted delivery of therapeutic oligonucleotides to tissues. Here, we present a protocol to generate, purify, and analyze AOCs from off-the-shelf antibodies. We describe steps to conjugate single/double-stranded oligonucleotides bearing amine handles to linkers and, then, to antibodies using well-established chemistry. In addition, we provide details regarding the purification techniques and analytical methods suitable for AOC. This protocol can be applied for several purposes where AOC is a modality of interest. For complete details on the use and execution of this protocol, please refer to Rady et al.<sup>1</sup>.
Authors
- Stéphane Erb (ORCID: https://orcid.org/0000-0001-7707-644X)
- Victor Lehot (ORCID: https://orcid.org/0000-0001-6557-7191)
- Julien Most
- Guilhem Chaubet (ORCID: https://orcid.org/0000-0001-6462-5112)
- Sarah Cianférani (ORCID: https://orcid.org/0000-0003-4013-4129)
- Nicolas Basse (ORCID: https://orcid.org/0000-0002-0009-601X)
- Tony Rady (ORCID: https://orcid.org/0000-0001-9692-0511)
- Alain Wagner (ORCID: https://orcid.org/0000-0003-3125-601X)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
- Sanofi (France) (FR)
- Institut de Recherche Interdisciplinaire de Grenoble (FR)
- Laboratoire de Chémo-biologie synthétique et thérapeutique (FR)
- Institut Polytechnique de Bordeaux (FR)
- Université de Strasbourg (FR)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-09-28
- DOI
- https://doi.org/10.1016/j.xpro.2024.103329
- Citations
- 4
- Primary Topic
- RNA Interference and Gene Delivery
- Type
- article
- Field-Weighted Citation Impact
- 0.94
Funders
- Sanofi
- Agence Nationale de la Recherche
- Association Nationale de la Recherche et de la Technologie
- Université de Strasbourg
- Centre National de la Recherche Scientifique