Obtaining, Optimizing, and Evaluating the Activity of Anticancer LfcinB-Derived Peptides on in vitro Models against Melanoma

Melanoma, a leading cause of cancer-related deaths, is expected to see a 50% increase in diagnoses and a 68% rise in mortality by 2040.This study focuses on evaluating and optimizing anticancer peptides derived from Bovine Lactoferricin and Buforin II in human (A-375) and murine (B16-F10) melanoma models.Peptide bioactivity was assessed using MTT assays and microscopy, followed by optimization through sequence modifications.Characterization was performed using RP-HPLC and LC-QTOF MS.The results of this study showed that the strategy of enhancement activity contributes to the development of new therapeutic agents for Melanoma treatment.