Designing self-assembling lipopeptide for tissue regeneration

Lipopetides are one class of biosurfactant materials, which have been widely studied in the past decade for tissue engineering applications.Their biocompatibility and flexibility make them ideal scaffold materials for tissue regeneration 1 .The hydrophobic lipid tail propels the self assembly and exposes the functional peptide sequences on the surface of the supramolecular structure.In our work, we've combined stearic acid with bioactive peptides which can imitate the structure and function of native ECM 2 .Here, the selfassembly propensity of these compounds are reported.The peptide sequences were synthesized using microwave assisted solid phase peptide synthesis technique on CEM liberty blue instrument. Stearic acid-REDV-NH 2The lipopeptides will be combined with low temperature gelling biopolymers like agarose to develop composite hydrogels which will then be used for 3D printing.The obtained scaffolds will be tested for their rheological properties and then seeded with L929 cells to evaluate their cytotoxicity and cell viability.