Magnetic nanobead assisted the dual targets driven fluorescent biosensor based on SPEXPAR and MNAzyme for the olfactory marker protein detection
Fig. 1: Schematic illustration of the principle of the Dual targets driven SPEXPAR assisted MNAzyme (D-S-M) fluorescent biosensor for olfactory marker protein (OMP) detection. A. Workflow for detection of OMP in nasal swab. B. Isothermal Self-Primer EXPonential Amplification Reaction (SPEXPAR) amplification. C. The production of fluorescent signal by Multicomponent Nuclear Acid Enzyme (MNAzyme). The signal of OMP was amplified and changed into the detectable fluorescence signal based on the reactions of SPEXPAR and MNAzyme in the D-S-M fluorescence biosensor. The qualitative or quantitative analysis of OMP can be measured by the analysis of the fluorescence intensity.Image 1.
Authors
- Jing Qi (ORCID: https://orcid.org/0000-0001-5020-0842)
- Feng Wang (ORCID: https://orcid.org/0000-0003-2980-4646)
- Hongyi Bao
- Yichen Wang
- Lin Chen (ORCID: https://orcid.org/0000-0002-7552-8673)
- Tuo‐Di Zhang
- Junling Yang (ORCID: https://orcid.org/0000-0001-7393-5171)
- Xuemin Cao
- Ya Wen (ORCID: https://orcid.org/0000-0002-1942-2110)
- Guixuan Ge
- Ping Wang (ORCID: https://orcid.org/0009-0008-2667-961X)
Institutions
- Nantong University (CN)
- Affiliated Hospital of Nantong University (CN)
- Suzhou Industrial Park Vocational Technical College (CN)
- ARTS Group (China) (CN)
- Zhejiang University (CN)
Publication Details
- Journal
- Materials Today Bio
- Published
- 2024-09-27
- DOI
- https://doi.org/10.1016/j.mtbio.2024.101272
- Primary Topic
- Advanced Chemical Sensor Technologies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Jiangsu Commission of Health
- National Natural Science Foundation of China
- Government of Jiangsu Province
- Nantong Science and Technology Bureau