The Effects of Anatolian Black Pine Cone Extracts on Carbonic Anhydrase Enzymes

Nowadays, there is an increasing trend in the use of natural products to treat diseases or alleviate their effects. This situation has emerged as a result of the side effect problem seen in the use of synthetic drugs. In this study, Anatolian black pine cone extracts were prepared using solvents of different polarities (water, methanol, ethanol, ethyl acetate, n-hexane). Then, the effects of these extracts on the esterase activities of hCA I and hCA II isoenzymes, which are associated with many physiological disorders, were examined in vitro. IC50 values were in the range of 5.10 to 49.50 μg/mL for hCA I and 2.57 to 15.07 μg/mL for hCA II. It can be said that these results are promising in terms of the potential of Anatolian black pine cone as a natural pharmacological agent.