Spectroscopic Determination of Taiopronin by Oxidative Coupling Method Using Dimethyl Phenylene Diamine

This article presents the spectrophotometric determination of taiopronin using an oxidative coupling reaction with dimethyl phenylene diamine. The UV-visible spectrophotometric method was developed to analyze taiopronin in pure drugs and pharmaceutical formulations. The process is easy to use, rapid, accurate, sensitive, and selective. It has the maximum absorption at 552 nm in wavelength. The method involves the combination of a drug solution with a reagent and an oxidizing agent, resulting in a purple solution that is dissolved in water and exhibits the highest absorption at a specific wavelength. The concentration range for the Beer's law is 5–30 μg/mL. The oxidation and conjugation process can be completed in two minutes at 25°C, with a stable absorption value for an hour. The process has high accuracy, linearity, and a recall rate of 99.42%. The detection limit, quantitative limit, and recovery rate are all high, demonstrating its precision and compatibility. The recovery studies generating up to 100% and a relative standard deviation of less than 2 demonstrated how accurate and precise the procedure was found to be, indicating excellent results. The proposed method has been effectively utilized in analyzing preparations that contain taiopronin.