HPTLC And FTIR Studies On Iridoid Glycoside (Negundoside) Isolated From Ethanol Leaf Extracts Of Vitex Negundo Linn.

In traditional Indian system of medicine Vitex negundo Linn is referred as Sarvaroganivarani -the remedy for all diseases.It is reported to possess wide range of medicinal uses and the ethanolic leaf extracts reported to contain flavanone, agnuside, casticin, herbacetin rhamnoside, kaempferol, luteolin-7-glucoside, negundoside, p-hydroxybenzoic acid, protocatechuic acid, quinic acid, vitedoin A, and vitexin.Investigation shows that negundoside is a potent phytopharmaceutical that acts in a novel way in inhibiting liver toxicity by interfering in the key events that are the main causative factors leading to liver dysfunction.This study aimed to isolate the phytoconstituent negundoside from ethanolic leaf extracts of Vitex negundo and identify the same by HPTLC fingerprinting and FTIR methods.The ethanol leaf extracts of Vitex negundo was subjected to column chromatography in a silica gel (60-120 mesh) glass column loaded onto the column of 46 2 cm and eluted with petroleum ether followed by cyclohexane, chloroform, ethyl acetate, methanol with increasing polarity.It resulted in six different fractions (A, B, C, D, E, F).The HPTLC chromatographic fingerprints for all fractions were recorded at 254 nm and 366 nm using the solvent system chloroform, methanol and acetic acid in the ratio 7:3:1.The results obtained for each fraction were compared with the HPTLC fingerprints and Rf values of reference standard negundoside developed under the same conditions.The lane graph and HPTLC fingerprint of isolated fraction E reported to have similar Rf value as compared with reference standard negundoside with varying displayed volumes.The FTIR spectrum of fraction E also showed peak wavelength values similar to that and reference standard negundoside.The present study offers valuable information and acts as a tool that can be used to identify negundoside from the ethanolic leaf extracts of Vitex negundo.