Structured illumination lensless digital holographic microscopy (SI-LDHM)

In this work, we propose a structured-illumination lensless digital holographic microscopy (SI-LDHM). SI-LDHM illuminates a sample with 24 structured illuminations (8 orientations × 3 phase shifts) and records the defocused interferogram formed by two copies of object waves along the ±1st diffraction orders of each SI. The reconstructed object waves under different illumination orientations are respectively propagated to the sample plane along the +1st diffraction order and then averaged, thus yielding a clean image without the artifact of twin images. Experimental results demonstrated that thanks to the multi-oriented SI strategy, the twin image in SI-LDHM is sevenfold reduced compared to conventional DHM, while the spatial resolution is 1.15 times higher.

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Publication Details

Journal
Frontiers in Physics
Published
2024-09-27
DOI
https://doi.org/10.3389/fphy.2024.1485687
Primary Topic
Digital Holography and Microscopy
Type
article
Field-Weighted Citation Impact
0.00

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article

Structured illumination lensless digital holographic microscopy (SI-LDHM)

Peng Gao, Juanjuan Zheng, Chao Zuo, Jiaming Qian et al.
Frontiers in Physics
Digital Holography and Microscopy
article

Structured illumination lensless digital holographic microscopy (SI-LDHM)

Peng Gao, Juanjuan Zheng, Chao Zuo, Jiaming Qian, Kai Wen, Sha An, Xiaofang Wang, Xuhong Guo, Ying Ma
article en

Abstract

In this work, we propose a structured-illumination lensless digital holographic microscopy (SI-LDHM). SI-LDHM illuminates a sample with 24 structured illuminations (8 orientations × 3 phase shifts) and records the defocused interferogram formed by two copies of object waves along the ±1st diffraction orders of each SI. The reconstructed object waves under different illumination orientations are respectively propagated to the sample plane along the +1st diffraction order and then averaged, thus yielding a clean image without the artifact of twin images. Experimental results demonstrated that thanks to the multi-oriented SI strategy, the twin image in SI-LDHM is sevenfold reduced compared to conventional DHM, while the spatial resolution is 1.15 times higher.

Frontiers in PhysicsVol. 12
Xidian University (CN), Nanjing University of Science and Technology (CN), Nanomaterials Research (United States) (US), Xi'an Institute of Optics and Precision Mechanics (CN)
National Natural Science Foundation of China, National Key Research and Development Program of China, Fundamental Research Funds for the Central Universities, Key Research and Development Projects of Shaanxi Province
Openalex Percentile: Top 85%
Digital Holography and Microscopy
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