Identification of Candida species from blood samples of neonates by PCR-RFLP method in Western U.P

Correct identification of Candida species is necessary for actual therapy and epidemiology study. species is the most frequent emerging cause of fungal infections globally. The most common cause of Candidemia worldwide is albicans, however, non- (NCA) species like being reported To identify the different species of from blood specimen of neonates by PCR-RFLP technique. Total 27 isolates were collected from blood samples of neonates. All the clinical specimens were inoculated for the isolation of species using standard mycological techniques. The isolates were cultured on Sabouraud’s Dextrose Agar (SDA) and further, they were identified by PCR and RFLP method. Molecular methods like PCR-RFLP were used in identifying six important species () from blood. By using universal primers ITS-1 and ITS-4, PCR assay successfully amplified the ITS-1 and ITS-2 region of 27 isolates and the amplicon size of approximately 510-870 bp. This method is speedy, easy, and affordable which can be used in routine laboratory diagnostics for the quick isolation of Candida species from blood samples. It is helpful for clinicians to start actual therapy in Candidemia patients before antifungal sensitivity results are obtainable.