Transcriptome and Metabolome Analysis of BmFAMeT6 Overexpression in Bombyx mori

<b>Background/Objectives:</b> The gene-encoding farnesyl diphosphate O-methyltransferase 6 (FAMeT 6) is a member of the farnesyl diphosphate O-methyltransferase family. Our previous studies have demonstrated its influence on juvenile hormone levels in third instar silkworm larvae. <b>Methods:</b> we utilized transcriptomic and metabolomic techniques to investigate the changes in third instar larvae at 0, 12, and 24 h following <i>BmFAMeT6</i> overexpression. <b>Results:</b> (1) The differentially expressed homologous genes were enriched in detoxification-related pathways at all three time points. (2) Transcription factor analysis of DEGs indicated a predominant presence of ZF-C2H2. (3) The metabolite-related network suggested that <i>BmFAMeT6</i> may influence the metabolism of silkworm larvae through the ABC transporters, purine metabolism, and tyrosine metabolism pathways. (4) The differential gene count, differential metabolite count, and types of metabolites at the three time points indicated a shift in the regulatory focus within the larvae as time progresses, with the inflection point of regulation occurring at the third instar larval stage, 12 h. <b>Conclusion:</b> In summary, our research indicates that the regulatory role of <i>BmFAMeT6</i> occurs within the context of the domestic silkworm's own growth and development regulation.