Prophage-encoded antibiotic resistance genes are enriched in human-impacted environments
The spread of antibiotic resistance genes (ARGs) poses a substantial threat to human health. Phage-mediated transduction could exacerbate ARG transmission. While several case studies exist, it is yet unclear to what extent phages encode and mobilize ARGs at the global scale and whether human impacts play a role in this across different habitats. Here, we combine 38,605 bacterial genomes, 1432 metagenomes, and 1186 metatranscriptomes across 12 contrasting habitats to explore the distribution of prophages and their cargo ARGs in natural and human-impacted environments. Worldwide, we observe a significant increase in the abundance, diversity, and activity of prophage-encoded ARGs in human-impacted habitats linked with relatively higher risk of past antibiotic exposure. This effect was driven by phage-encoded cargo ARGs that could be mobilized to provide increased resistance in heterologous E. coli host for a subset of analyzed strains. Our findings suggest that human activities have altered bacteria-phage interactions, enriching ARGs in prophages and making ARGs more mobile across habitats globally.
Authors
- Hanpeng Liao (ORCID: https://orcid.org/0000-0001-7539-2668)
- Steven W. Wilhelm (ORCID: https://orcid.org/0000-0001-6283-8077)
- Zhong Wei (ORCID: https://orcid.org/0000-0002-7967-4897)
- Mark Radosevich (ORCID: https://orcid.org/0000-0001-9162-8036)
- Shungui Zhou (ORCID: https://orcid.org/0000-0003-0899-4225)
- Michael R. Gillings (ORCID: https://orcid.org/0000-0002-4043-4351)
- Chaofan Ai
- Xiaolong Liang (ORCID: https://orcid.org/0000-0002-3759-3976)
- Ville‐Petri Friman (ORCID: https://orcid.org/0000-0002-1592-157X)
- Brajesh K. Singh (ORCID: https://orcid.org/0000-0003-4413-4185)
- Qiue Yang (ORCID: https://orcid.org/0000-0002-0552-7763)
- Manuel Delgado‐Baquerizo (ORCID: https://orcid.org/0000-0002-6499-576X)
- David J. Eldridge (ORCID: https://orcid.org/0000-0002-2191-486X)
- Chen Liu (ORCID: https://orcid.org/0009-0003-0430-6146)
- Yong‐Guan Zhu (ORCID: https://orcid.org/0000-0003-3861-8482)
- Xiang Tang (ORCID: https://orcid.org/0000-0001-6764-3447)
- Chunqin Liu (ORCID: https://orcid.org/0000-0001-8851-189X)
Institutions
- Nanjing Agricultural University (CN)
- University of Helsinki (FI)
- Chinese Academy of Sciences (CN)
- UNSW Sydney (AU)
- Institute of Applied Ecology (CN)
- ARC Centre of Excellence in Synthetic Biology (AU)
- Instituto de Recursos Naturales y Agrobiología de Sevilla (ES)
- Research Center for Eco-Environmental Sciences (CN)
- Fujian Agriculture and Forestry University (CN)
- Western Sydney University (AU)
- University of Tennessee at Knoxville (US)
- Macquarie University (AU)
Publication Details
- Journal
- Nature Communications
- Published
- 2024-09-27
- DOI
- https://doi.org/10.1038/s41467-024-52450-y
- Citations
- 77
- Primary Topic
- Bacteriophages and microbial interactions
- Type
- article
- Field-Weighted Citation Impact
- 35.33
Funders
- National Natural Science Foundation of China
- Novo Nordisk
- Ministerio de Ciencia e Innovación
- Agencia Estatal de Investigación