Developing 1,4-Diethyl-1,2,3,4-tetrahydroquinoxalin-substituted Fluorogens Based on GFP Chromophore for Endoplasmic Reticulum and Lysosome Staining
In the present study, we demonstrated that the introduction of a 1,4-diethyl-1,2,3,4-tetrahydroquinoxalin moiety into the arylidene part of GFP chromophore-derived compounds results in the formation of environment-sensitive fluorogens. The rationally designed and synthesized compounds exhibit remarkable solvent- and pH-dependence in fluorescence intensity. The solvent-dependent variation in fluorescence quantum yield makes it possible to use some of the proposed compounds as polarity sensors suitable for selective endoplasmic reticulum fluorescent labeling in living cells. Moreover, the pH-dependent emission intensity variation of other fluorogens makes them selective fluorescent labels for the lysosomes in living cells.
Authors
- Михаил С. Баранов (ORCID: https://orcid.org/0000-0002-9339-7603)
- Анатолий И. Соколов
- Alexander S. Mishin (ORCID: https://orcid.org/0000-0002-4935-7030)
- Ivan N. Myasnyanko (ORCID: https://orcid.org/0000-0002-2168-3555)
- Chong Fang (ORCID: https://orcid.org/0000-0002-8879-1825)
- Maxim M. Perfilov (ORCID: https://orcid.org/0000-0002-8987-9562)
- Надежда С. Балеева (ORCID: https://orcid.org/0000-0003-2737-0733)
- D. I. Rudik (ORCID: https://orcid.org/0009-0004-1607-6365)
- Cheng Chen (ORCID: https://orcid.org/0000-0003-0561-5226)
- Yulia A. Bogdanova (ORCID: https://orcid.org/0000-0003-1745-4204)
Institutions
- Peoples' Friendship University of Russia (RU)
- Oregon State University (US)
- Pirogov Russian National Research Medical University (RU)
- Institute of Bioorganic Chemistry (RU)
Publication Details
- Journal
- International Journal of Molecular Sciences
- Published
- 2024-09-27
- DOI
- https://doi.org/10.3390/ijms251910448
- Citations
- 2
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.47
Funders
- Ministry of Health of the Russian Federation