Discovery of Novel Inhibitors against ALS-Related SOD1(A4V) Aggregation through the Screening of a Chemical Library Using Differential Scanning Fluorimetry (DSF)
Dimer stability was the parameter chosen as the criterion for screening, since the dissociation of the native SOD1 dimer is the step prior to its in vitro aggregation. The screening revealed one compound raising protein-ligand T<sub>m</sub> by 6 °C, eleven inducing a higher second T<sub>m</sub>, suggesting a stabilization effect, and fourteen reducing T<sub>m</sub> from 10 up to 26 °C, suggesting possible interactions or non-specific binding.
Authors
- Vassilios Myrianthopoulos (ORCID: https://orcid.org/0000-0001-8359-9634)
- Constantinos E. Vorgias (ORCID: https://orcid.org/0000-0002-0909-9401)
- Μαρία Γιαννάκου (ORCID: https://orcid.org/0000-0003-0282-1585)
- Ifigeneia Akrani
- Emmanuel Mikros (ORCID: https://orcid.org/0000-0002-5984-5169)
- Dimitris G. Hatzinikolaou (ORCID: https://orcid.org/0000-0003-1674-8518)
- Angeliki Tsoka
Institutions
- National and Kapodistrian University of Athens (GR)
Publication Details
- Journal
- Pharmaceuticals
- Published
- 2024-09-27
- DOI
- https://doi.org/10.3390/ph17101286
- Citations
- 4
- Primary Topic
- Amyotrophic Lateral Sclerosis Research
- Type
- article
- Field-Weighted Citation Impact
- 1.48
Funders
- European Commission
- State Scholarships Foundation
- European Social Fund