1H-NMR Spectroscopy of Blood Plasma for Detection of Changes in Metabolism during the Development of Sarcoma M-1 in Rats

An assessment of the effectiveness of 1H NMR-based metabolomic analysis for the detection of metabolic changes during carcinogenesis was made based on a comparison of the quantitative composition of metabolites in the blood plasma of rats for healthy rats and rats receiving M-1 sarcoma transplantation. Plasma was collected from the rats under study on day 12 and day 36 after sarcoma transplantation to identify metabolites associated with tumor development. Analysis of NMR spectra using multivariate statistical methods showed differences in the composition of metabolites for the groups of animals under study already on day 12 after sarcoma transplantation; on day 36 the differences were significant. 23 metabolites were quantified. On day 12, only the lactate and allantoin levels were significantly different between the groups, while on day 36, the levels of 9 metabolites were different in rats in all groups. All of the metabolites identified are involved in cancer metabolism, which makes 1H NMR spectroscopy a promising method for cancer diagnosis.