The Nature of Intermolecular Interactions Affecting Oligomerization of Nt.BspD6I Nickase

Knowledge of the nature of intermolecular interactions and amino acid residues unveiling their origin is necessary to enable alteration of the stability of intermolecular complexes. In this work, using Nt.BspD6I nickase as an example, it was shown that hydrophobic interactions have an influence on the protein’s ability to oligomerization and that chemical and geometric complementarity of external surfaces is a necessary condition for the formation of stable homo complexes.