Increased Drug Resistance in Acute Lymphoblastic Leukemia Cells in Three-Dimensional High-Density Cell Cultures

In this study, the development process of drug resistance in acute lymphoblastic leukemia cells (Jurkat, MOLT 3, and MOLT 4 cell lines) was examined in high-density cell cultures. It has been shown that in highdensity cultures of acute lymphoblastic leukemia cells resistance to the action of chemotherapeutic drugs increases comparing to the cells cultured under low-density conditions. The results obtained after investigation of the mechanism underlying increased drug resistance in acute lymphoblastic leukemia cells in high-density cell cultures showed that an increase in drug resistance in high-density cultures of cells can be mediated by a change in their proliferative activity. These findings can be applied in developing a strategy to overcome drug resistance in acute lymphoblastic leukemia cells, which depends on the density of the cell culture.