Buffer-free high pH mobile phase LC-MS/MS for determination of the alcohol biomarker phosphatidylethanol 16:0/18:1 and 20 drugs and metabolites in whole blood
An LC-MS/MS method for simultaneous determination of PEth 16:0/18:1 and 20 drugs and metabolites in whole blood were for the first time developed and validated. Retention of PEth 16:0/18:1 was, in contrast to the other 20 compounds, largely affected by mobile phase buffer concentration. The buffer free basic mobile phase ensured that phosphatidylethanol 16:0/18:1 eluted before most of the unwanted phospholipids.
Authors
- Galina Nilsson
- Benedicte Jørgenrud (ORCID: https://orcid.org/0000-0001-5917-8776)
- Tao Angell‐Petersen McQuade
- Marisa Maria (ORCID: https://orcid.org/0000-0001-5171-3877)
- Thomas Berg (ORCID: https://orcid.org/0000-0002-7675-2095)
Institutions
- Oslo University Hospital (NO)
- University of Lisbon (PT)
Publication Details
- Journal
- Talanta
- Published
- 2024-09-27
- DOI
- https://doi.org/10.1016/j.talanta.2024.126964
- Citations
- 3
- Primary Topic
- Alcohol Consumption and Health Effects
- Type
- article
- Field-Weighted Citation Impact
- 1.27
Funders
- Fundação para a Ciência e a Tecnologia