Electrodeposited ZnO Nanostructures on ITO Surfaces: Exploring Their Efficacy for Cholesterol Biosensing Applications

In this study, ZnO nanostructures were prepared by electrochemical anodization of electrodeposited Zn on ITO/glass substrates for cholesterol detection. The efficiency of the developed ZnO nanostructures in the detection of the Cholesterol oxidase (ChOx) enzyme was determined by the cyclic voltammetry method. The XRD and SEM results confirmed the synthesis of ZnO nanostructures prepared by the anodization method with various parameters. The effect of electrodeposition and anodization time on the morphology was observed. Cyclic voltammetry of ZnO/Zn/ITO/glass and Pt/ZnO/Zn/ITO/glass electrodes in electrolytes with various cholesterol concentrations was performed. The detection limit of the obtained Pt/ZnO/Zn/ITO/glass structured electrode was calculated as 0.965x10-3M. The resulting material with a layered structure may have potential applications in electrochemical sensors and biosensors in biomedical applications. In addition to biosensing performance, this study proposes a new approach for the development of ZnO-based biosensors that does not require expensive infrastructure and raw material costs, making it possible to develop high-sensitivity biosensor electrodes with lower detection limits with improvements to be made in future studies.