Rapid screening of Salmonella typhimurium based on a bacterial surface-imprinted polymer-functionalized bipolar electrode-electrochemiluminescence platform

A bipolar electrode-electrochemiluminescence (BPE-ECL) sensor, utilizing the distinctive properties of a polydopamine (PDA) bacterial surface-imprinted polymer (SIP), has been skillfully developed for the highly sensitive detection of Salmonella typhimurium ( S. typhimurium). • A BPE-ECL platform based on bacterial surface imprinted polymer was constructed for S. typhimurium detection. • Fc-aptamer- S. typhimurium as a signal probe specifically recognized S. typhimurium. • A low detection limit of 10 CFU mL −1 S. typhimurium could be identified. A bipolar electrode-electrochemiluminescence (BPE-ECL) sensor, utilizing a polydopamine (PDA) bacterial surface-imprinted polymer (SIP), has been developed for the detection of Salmonella typhimurium ( S. typhimurium). To fabricate this sensor, dopamine (DA) served as the monomer, while S. typhimurium played a crucial role as the template molecule. Through a precision-tuned electropolymerization process, a polymer film template was seamlessly crafted on the cathode of BPE, encasing the target bacteria within its matrix. Once the template was delicately removed, a molecular surface-imprinted film emerged on the cathode, forming a PDA SIP capture probe that was remarkably adept at recognizing and binding to the target bacteria. When the target bacteria was present on the cathode, the Fc-aptamer- S. typhimurium acted as a signal probe to specifically recognized S. typhimurium. Applying a constant potential to the BPE, the oxidation product Fc + , generated at the cathode, accelerating the luminescence of the [Ru(bpy) 3 ] 2+ /TPrA system on the anode. The sensor exhibited a remarkable sensitivity, capable of detecting S. typhimurium concentrations ranging from 10 1 to 10 6 CFU mL −1 , with a detection limit of 10 CFU mL −1 .