Protocol for the acquisition and maturation of oligodendrocytes from neonatal rodent brains
The generation of an oligodendrocyte primary culture model encompassing the diverse stages of the lineage is essential for the in vitro research of oligodendrocyte physiology and pathophysiology. Here, we provide a protocol for generating oligodendrocytes from the neonatal rodent brain. We describe steps for isolating oligodendrocyte progenitor cells (OPCs) through differential centrifugation, their subsequent expansion, passaging, and differentiation. For complete details on the use and execution of this protocol, please refer to Kim et al.<sup>1</sup>.
Authors
- Jun Young Choi (ORCID: https://orcid.org/0000-0002-6031-3549)
- Hanki Kim (ORCID: https://orcid.org/0000-0003-2568-4472)
- Byung Gon Kim (ORCID: https://orcid.org/0000-0003-2233-9569)
- Xuelian Jin
- Hyo Jin Cho (ORCID: https://orcid.org/0000-0001-5261-0693)
- Seungyon Koh (ORCID: https://orcid.org/0000-0002-5547-6378)
- Bum‐Joon Kim (ORCID: https://orcid.org/0000-0003-0085-6709)
Institutions
- Ajou University (KR)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-09-27
- DOI
- https://doi.org/10.1016/j.xpro.2024.103327
- Primary Topic
- Cell Image Analysis Techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Research Foundation
- Korea Health Industry Development Institute
- National Research Foundation of Korea
- Ministry of Science and ICT, South Korea