Isolating Sensory Neurons and Co-Culturing with Natural Killer Cells

Source:Ahmadi, A., et al. Teasing out the interplay between natural killer cells and nociceptor neurons. J. Vis. Exp. (2022). This video describes the isolation of DRG neurons through enzymatic digestion, mechanical dissociation, and gradient centrifugation. The DRG cells are co-cultured with natural killer cells on an extracellular matrix to study the interaction between immune cells and sensory neurons.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2024-09-27
DOI
https://doi.org/10.3791/22632
Primary Topic
Immune Cell Function and Interaction
Type
article
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article

Isolating Sensory Neurons and Co-Culturing with Natural Killer Cells

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Journal of Visualized Experiments
Immune Cell Function and Interaction
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Isolating Sensory Neurons and Co-Culturing with Natural Killer Cells

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Abstract

Source:Ahmadi, A., et al. Teasing out the interplay between natural killer cells and nociceptor neurons. J. Vis. Exp. (2022). This video describes the isolation of DRG neurons through enzymatic digestion, mechanical dissociation, and gradient centrifugation. The DRG cells are co-cultured with natural killer cells on an extracellular matrix to study the interaction between immune cells and sensory neurons.

Journal of Visualized Experiments
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Immune Cell Function and Interaction
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