Development and Maintenance of Serum-Free Embryoid Bodies
Source: Phillips, A. W., et al. Developing HiPSC Derived Serum Free Embryoid Bodies for the Interrogation of 3-D Stem Cell Cultures Using Physiologically Relevant Assays. J. Vis. Exp. (2017). The video describes the procedure for developing serum-free embryoid bodies (SFEBs) from neuronal progenitor cells using various serum-free differentiation mediums or DM. DM-2 facilitates neurons' maturation and arrangement to form SFEBs, while DM-3 is used for further growth maintenance.
Authors
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Publication Details
- Journal
- Journal of Visualized Experiments
- Published
- 2024-09-27
- DOI
- https://doi.org/10.3791/22636
- Primary Topic
- Reproductive Biology and Fertility
- Type
- article
- Field-Weighted Citation Impact
- 0.00