Dissection and Mounting of Fruit Fly Larval Brain Explants

Source: Sheng, C., et al. Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons. J. Vis. Exp. (2019). This video demonstrates the dissection and mounting of brain explants from Drosophila larvae. The central nervous system (CNS), attached to eye disks, is extracted from the larvae and then mounted on a glass slide in a physiological saline solution.