Establishing Primary Mixed Glial Cell Cultures from a Mouse Spinal Cord

Source: Malon, J. T., et al. Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue. J. Vis. Exp. (2016). This video demonstrates a technique for isolating and culturing primary mixed glial cells from adult mouse spinal cord tissue. The protocol involves enzymatic digestion of the tissue, followed by the removal of broken cell membranes and myelin debris to obtain a single-cell population. The cells are then cultured in media optimized for mixed glial cell growth.