Isolating Murine Retinal Ganglion Cells Using Fluorescence-Activated Cell Sorting

Source: Chintalapudi, S. R., et al. Isolation of Primary Murine Retinal Ganglion Cells (RGCs) by Flow Cytometry. J. Vis. Exp. (2017) This video demonstrates the procedure for isolating murine primary retinal ganglion cells (RGCs) by using flow cytometry. This method allows for the future study of RGCs, with the goal of better understanding the major decline in visual acuity that results from the loss of functional RGCs in neurodegenerative diseases.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2024-09-27
DOI
https://doi.org/10.3791/22621
Primary Topic
Retinal Development and Disorders
Type
article
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article

Isolating Murine Retinal Ganglion Cells Using Fluorescence-Activated Cell Sorting

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Journal of Visualized Experiments
Retinal Development and Disorders
article

Isolating Murine Retinal Ganglion Cells Using Fluorescence-Activated Cell Sorting

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Abstract

Source: Chintalapudi, S. R., et al. Isolation of Primary Murine Retinal Ganglion Cells (RGCs) by Flow Cytometry. J. Vis. Exp. (2017) This video demonstrates the procedure for isolating murine primary retinal ganglion cells (RGCs) by using flow cytometry. This method allows for the future study of RGCs, with the goal of better understanding the major decline in visual acuity that results from the loss of functional RGCs in neurodegenerative diseases.

Journal of Visualized Experiments
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Retinal Development and Disorders
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