Molecular characterization, virulence and antimicrobial and biocidal susceptibility of selected bacteria isolated from the cloaca of nestling ospreys (Pandion haliaetus) from Mono Lake, California, USA
In the present study, the presence of the Enterobacterales, Staphylococcus spp., Mammaliicoccus spp., and Enterococcus spp. in cloacal samples of nestling ospreys (Pandion haliaetus), a fish-eating specialist, from Mono Lake, California, USA was examined by a multiphasic approach, including antimicrobial and biocide susceptibility testing, genotyping, and whole genome sequencing of selected isolates. The most commonly detected species was Escherichia coli, followed by Mammaliicoccus sciuri, Staphylococcus delphini, Enterococcus faecalis, Enterococcus faecium, Hafnia alvei, Klebsiella pneumoniae, Citrobacter braakii and single isolates of Edwardsiella tarda, Edwardsiella albertii, Klebsiella aerogenes, Plesiomonas shigelloides and Staphylococcus pseudintermedius. Multi-drug resistance (MDR) was observed in two E. coli isolates and in an Enterococcus faecium isolate. The MDR blaCTX-M-55-positive E. coli belonged to the pandemic clone ST58. The results of the present study suggest that nestling ospreys are exposed to MDR bacteria, possibly through the ingestion of contaminated fish. Ospreys may be good biosentinels for the presence of these microorganisms and antibiotic resistance in the local environment and the risk for other wildlife, livestock and humans.
Authors
- Peter Bloom (ORCID: https://orcid.org/0000-0003-2225-6149)
- Adriana Cabal Rosel (ORCID: https://orcid.org/0000-0001-9346-1612)
- Stefan Monecke (ORCID: https://orcid.org/0000-0003-4368-8709)
- Štefan Schwarz (ORCID: https://orcid.org/0000-0002-6303-8212)
- Miguel D. Saggese (ORCID: https://orcid.org/0000-0002-9701-6708)
- Igor Loncaric (ORCID: https://orcid.org/0000-0003-1401-2961)
- Olivia M. Grünzweil
- Andrea T. Feßler (ORCID: https://orcid.org/0000-0002-0510-7347)
- Joachim Spergser (ORCID: https://orcid.org/0000-0002-0164-0179)
- Werner Ruppitsch (ORCID: https://orcid.org/0000-0001-9940-3333)
- Dušan Mišić (ORCID: https://orcid.org/0000-0001-5393-8922)
- Ralf Ehricht (ORCID: https://orcid.org/0000-0002-6612-0043)
- Michael P. Szostak (ORCID: https://orcid.org/0000-0002-3336-6434)
- Elke Müller
- Sascha D. Braun
- Alina Riegelnegg
- Ashli Lewis
Institutions
- California Department of Parks and Recreation (US)
- University of Veterinary Medicine Vienna (AT)
- Wrocław University of Environmental and Life Sciences (PL)
- Leibniz Institute of Photonic Technology (DE)
- Institut für Medizinische Informatik, Biometrie und Epidemiologie (DE)
- OFM Research (United States) (US)
- InfectoGnostics Research Campus Jena (DE)
- Applied Design Labs (United States) (US)
- Klinik und Poliklinik für Psychotherapie und Psychosomatik (DE)
- Austrian Agency for Health and Food Safety (AT)
- Freie Universität Berlin (DE)
- Friedrich Schiller University Jena (DE)
- Technische Universität Dresden (DE)
- Western University of Health Sciences (US)
Publication Details
- Journal
- PLoS ONE
- Published
- 2024-09-27
- DOI
- https://doi.org/10.1371/journal.pone.0311306
- Citations
- 3
- Primary Topic
- Microbial infections and disease research
- Type
- article
- Field-Weighted Citation Impact
- 1.03