An in vivo tumour organoid model based on the chick embryonic chorioallantoic membrane mimics key characteristics of the patient tissue: a proof-of-concept study
We successfully established an in vivo CAM-PDX model based on CRC PDOs. The histomorphological features and target protein expression of the original patient's tissue were mirrored in the in vitro PDOs, and particularly in the in vivo CAM-PDXs. The [<sup>68</sup>Ga]Ga-Pentixafor uptake patterns were comparable between in vitro, in ovo and clinical data and 2-[<sup>18</sup>F]FDG was avidly taken up in the patient's liver metastasis and CAM-PDXs. We thus propose the CAM-PDX model as an alternative in vivo model with promising translational value for CRC patients.
Authors
- Thomas H. Helbich (ORCID: https://orcid.org/0000-0003-3169-778X)
- Alexander Haug (ORCID: https://orcid.org/0000-0002-8308-6174)
- Joachim Friske (ORCID: https://orcid.org/0000-0002-1830-8113)
- Markus Zeitlinger (ORCID: https://orcid.org/0000-0002-1873-3953)
- Michael Bergmann (ORCID: https://orcid.org/0000-0001-8529-1166)
- Markus Mitterhauser (ORCID: https://orcid.org/0000-0003-3173-5272)
- Theresa Balber (ORCID: https://orcid.org/0000-0002-8154-5680)
- Loan Tran
- Gerda Egger (ORCID: https://orcid.org/0000-0003-2489-155X)
- Katarína Benčurová (ORCID: https://orcid.org/0000-0001-6762-0528)
- Kajetana Bevc
- Marcus Hacker (ORCID: https://orcid.org/0000-0002-4222-4083)
Institutions
- University of Vienna (AT)
- Comprehensive Cancer Center Vienna (AT)
- Christian Doppler Laboratory for Thermoelectricity (AT)
- Ludwig Boltzmann Institute Applied Diagnostics (AT)
- Medical University of Vienna (AT)
Publication Details
- Journal
- EJNMMI Research
- Published
- 2024-09-27
- DOI
- https://doi.org/10.1186/s13550-024-01151-0
- Citations
- 5
- Primary Topic
- Cancer Cells and Metastasis
- Type
- article
- Field-Weighted Citation Impact
- 1.41
Funders
- Universität Wien
- Medizinische Universität Wien